pEBB modified
(Plasmid #22226)

• Purpose: (Empty Backbone)
• Depositing Lab: David Baltimore
• Publication: Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37.

Backbone

• Vector backbone: pEF-Bos
• Backbone size (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstXI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector was the backbone for several of the Ananda Roy plasmids.

SpeI and KpnI site are missing from the MCS, unlike the original Baltimore vector.