FCK-ss-Prl-Arch-GFP
(Plasmid #22224)

• Purpose: Lentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.
• Depositing Lab: Edward Boyden
• Publication: Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Backbone

• Vector backbone: FCK(1.3)GW
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9227
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ss-Prl-Arch-GFP
• Alt name: Arch
• Alt name: archaerhodopsin-3
• Alt name: H. sodomense archaerhodopsin-3
• Species: H. sodomense
• Insert Size (bp): 1689
• Promoter: CaMKII
• Tags / Fusion Proteins:
  • ss (N terminal on insert)
  • Prl (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: atgactgagaccctcccacccgtg actgaaagcgccgt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.