pcDNA-DDX53-GFP
(Plasmid
#222239)
-
PurposeExpress GFP tagged DDX53 in mammalian cells
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 222239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA™6.2/C-EmGFP-DEST
- Backbone size w/o insert (bp) 7442
- Total vector size (bp) 7900
-
Vector typeMammalian Expression
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDDX53
-
Alt nameCAGE
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1900
-
Entrez GeneDDX53 (a.k.a. CAGE, CT26)
- Promoter CMV and T7
-
Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV promoter (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA-DDX53-GFP was a gift from Yuliang Wu (Addgene plasmid # 222239 ; http://n2t.net/addgene:222239 ; RRID:Addgene_222239)