FCK-Mac-GFP
(Plasmid #22223)

• Purpose: Lentiviral expression of Mac-GFP for optogenetic inhibition
• Depositing Lab: Edward Boyden
• Publication: Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Backbone

• Vector backbone: FCK(1.3)GW
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9227
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mac
• Alt name: Mac-GFP
• Alt name: Leptosphaeria maculans opsin
• Alt name: opsin
• Species: L. maculans
• Insert Size (bp): 1683
• Mutation: NO
• Promoter: CaMKII
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: atgactgagaccctcccacccgtg actgaaagcgccgt

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.