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Addgene

pSCL194
(Plasmid #222225)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222225 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZS.157
  • Vector type
    Yeast Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    site 1/Cas9/Eco1 RT
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCL194 was a gift from Seth Shipman (Addgene plasmid # 222225 ; http://n2t.net/addgene:222225 ; RRID:Addgene_222225)

  • For your References section:

    High Throughput Variant Libraries and Machine Learning Yield Design Rules for Retron Gene Editors. Crawford KD, Khan AG, Lopez SC, Goodarzi H, Shipman S. bioRxiv [Preprint]. 2024 Jul 9:2024.07.08.602561. doi: 10.1101/2024.07.08.602561. 10.1101/2024.07.08.602561 PubMed 39026735
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