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Addgene

pAGK43
(Plasmid #222223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSCL273
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Eco1 RT; 112 nt donor; PAM -19
  • gRNA/shRNA sequence
    TCTGGGGTAAAGGCTGTA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6932
  • Entrez Gene
    NPAS2 (a.k.a. MOP4, PASD4, bHLHe9)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGK43 was a gift from Seth Shipman (Addgene plasmid # 222223 ; http://n2t.net/addgene:222223 ; RRID:Addgene_222223)

  • For your References section:

    High Throughput Variant Libraries and Machine Learning Yield Design Rules for Retron Gene Editors. Crawford KD, Khan AG, Lopez SC, Goodarzi H, Shipman S. bioRxiv [Preprint]. 2024 Jul 9:2024.07.08.602561. doi: 10.1101/2024.07.08.602561. 10.1101/2024.07.08.602561 PubMed 39026735
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