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Addgene

pSN95
(Plasmid #222220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD444-CH
  • Backbone size w/o insert (bp) 4037
  • Total vector size (bp) 5083
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vanA putida
  • Alt name
    PP_3736
  • Species
    Pseudomonas putida KT2440
  • Insert Size (bp)
    1068
  • GenBank ID
    AAN69332.1
  • Entrez Gene
    PP_RS19450 (a.k.a. PP_RS19450, PP_3736)
  • Promoter T5-lac
  • Tag / Fusion Protein
    • 6x His (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSN95 was a gift from Gregg Beckham (Addgene plasmid # 222220 ; http://n2t.net/addgene:222220 ; RRID:Addgene_222220)

  • For your References section:

    Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440. Bleem AC, Kuatsjah E, Johnsen J, Mohamed ET, Alexander WG, Kellermyer ZA, Carroll AL, Rossi R, Schlander IB, Peabody V GL, Guss AM, Feist AM, Beckham GT. Metab Eng. 2024 Jul;84:145-157. doi: 10.1016/j.ymben.2024.06.009. Epub 2024 Jun 25. 10.1016/j.ymben.2024.06.009 PubMed 38936762
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