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pACB143
(Plasmid #222214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6154
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vanB(A24P)
  • Alt name
    PP_3737
  • Species
    Pseudomonas putida KT2440
  • Insert Size (bp)
    894
  • Mutation
    Changed Alanine 24 to Proline
  • GenBank ID
    AAN69333.1
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal, thrombin-cleavable 6x His tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACB143 was a gift from Gregg Beckham (Addgene plasmid # 222214 ; http://n2t.net/addgene:222214 ; RRID:Addgene_222214)

  • For your References section:

    Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440. Bleem AC, Kuatsjah E, Johnsen J, Mohamed ET, Alexander WG, Kellermyer ZA, Carroll AL, Rossi R, Schlander IB, Peabody V GL, Guss AM, Feist AM, Beckham GT. Metab Eng. 2024 Jul;84:145-157. doi: 10.1016/j.ymben.2024.06.009. Epub 2024 Jun 25. 10.1016/j.ymben.2024.06.009 PubMed 38936762
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