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pET20b-mPTER-Y65F
(Plasmid #222203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-20b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli BL21 should be used for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTER
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1113
  • Mutation
    Y65F
  • Entrez Gene
    Pter (a.k.a. Mpr56-1)
  • Tags / Fusion Proteins
    • Strep (N terminal on insert)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.21.586194 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b-mPTER-Y65F was a gift from Jonathan Long (Addgene plasmid # 222203 ; http://n2t.net/addgene:222203 ; RRID:Addgene_222203)

  • For your References section:

    A PTER-dependent pathway of taurine metabolism linked to energy balance. Wei W, Lyu X, Markhard AL, Fu S, Mardjuki RE, Cavanagh PE, Zeng X, Rajniak J, Lu N, Xiao S, Zhao M, Moya-Garzon MD, Truong SD, Chou JC, Wat LW, Chidambaranathan-Reghupaty S, Coassolo L, Xu D, Shen F, Huang W, Ramirez CB, Jang C, Svensson KJ, Fischbach MA, Long JZ. bioRxiv [Preprint]. 2024 Mar 22:2024.03.21.586194. doi: 10.1101/2024.03.21.586194. 10.1101/2024.03.21.586194 PubMed 38562797
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