pcDNA3.1V5-His Snk/Plk2 D223N
(Plasmid #22218)

• Depositing Lab: Wafik El-Deiry
• Publication: Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71.

Backbone

• Vector backbone: pcDNA3.1/V5-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Snk/Plk2
• Alt name: Snk
• Alt name: Plk2
• Species: H. sapiens (human)
• Mutation: single amino acid substitution mutant, aspartic acid 223 to asparagine
• Entrez Gene: PLK2 (a.k.a. SNK, hPlk2, hSNK)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human Snk/Plk2 was amplified from a human fetal brain cDNA library (Clontech) using PFU Turbo (Invitrogen) using the following primers: 5' CGC GGA TCC GCG ACC ATG GAG CTT TTG 3' and 5' CCG CTC GAG GTT ACA TCT TTG TAA GAG CAT 3'.