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pcs2+spCas9 ABE-Umax-nanos
(Plasmid #222142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Total vector size (bp) 9541
  • Vector type
    CRISPR ; Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Modified TadA-nSpCas9 with nanos UTR
  • Species
    Synthetic
  • Promoter SP6
  • Tags / Fusion Proteins
    • Bipartite NLS (N terminal on insert)
    • Bipartite NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use SP6 for in vitro transcription

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcs2+spCas9 ABE-Umax-nanos was a gift from Gaurav Varshney (Addgene plasmid # 222142 ; http://n2t.net/addgene:222142 ; RRID:Addgene_222142)

  • For your References section:

    ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish. Qin W, Liang F, Lin SJ, Petree C, Huang K, Zhang Y, Li L, Varshney P, Mourrain P, Liu Y, Varshney GK. Nat Commun. 2024 Jul 4;15(1):5613. doi: 10.1038/s41467-024-49943-1. 10.1038/s41467-024-49943-1 PubMed 38965236
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