pT3TS-spCas9 ABE-Umax
(Plasmid
#222138)
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PurposeAdenine base editor with modified TadA variant for A-to-G base editing in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT3TS
- Backbone size w/o insert (bp) 2800
- Total vector size (bp) 7978
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Vector typeCRISPR ; Zebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameModified TadA-nSpCas9
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SpeciesSynthetic
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GenBank ID
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Entrez Genecas9 (a.k.a. SD90_RS03825, SD90_03830)
- Promoter T3
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Tags
/ Fusion Proteins
- bipartite NLS (N terminal on insert)
- bipartite NLS (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer aattaaccctcactaaagg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT3TS-spCas9 ABE-Umax was a gift from Gaurav Varshney (Addgene plasmid # 222138 ; http://n2t.net/addgene:222138 ; RRID:Addgene_222138) -
For your References section:
ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish. Qin W, Liang F, Lin SJ, Petree C, Huang K, Zhang Y, Li L, Varshney P, Mourrain P, Liu Y, Varshney GK. Nat Commun. 2024 Jul 4;15(1):5613. doi: 10.1038/s41467-024-49943-1. 10.1038/s41467-024-49943-1 PubMed 38965236