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pEIF3D-RNAgateDel-sgEIF3D
(Plasmid #222137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMJ179
  • Backbone manufacturer
    Jonathan Weissman, Addgene plasmid #85996
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF3D
  • Species
    H. sapiens (human)
  • Mutation
    V291-F306 deletion
  • Entrez Gene
    EIF3D (a.k.a. EIF3S7, eIF3-p66, eIF3-zeta)
  • Tag / Fusion Protein
    • BFP-P2A (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene observed 3 mismatches in the insert leading to 1 silent mutation and 2 substitutions (N772K, G792W). These mutations have been confirmed to not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEIF3D-RNAgateDel-sgEIF3D was a gift from Jonathan Weissman (Addgene plasmid # 222137 ; http://n2t.net/addgene:222137 ; RRID:Addgene_222137)

  • For your References section:

    Translational fidelity screens in mammalian cells reveal eIF3 and eIF4G2 as regulators of start codon selectivity. She R, Luo J, Weissman JS. Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 10.1093/nar/gkad329. 10.1093/nar/gkad329 PubMed 37144468
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