pEGFPC1-muscle Amphiphysin 2
(Plasmid #22213)

• Depositing Lab: Pietro De Camilli
• Publication: Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Amphiphysin II
• Alt name: BIN1
• Alt name: SH3P9
• Species: H. sapiens (human)
• Insert Size (bp): 1998
• GenBank ID: U87558
• Entrez Gene: BIN1 (a.k.a. AMPH2, AMPHL, CNM2, SH3P9)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII/ BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primere
• 3′ sequencing primer: EGFP-C Sequencing Primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry