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Addgene

pGFP-AUC-IRES-mCherry
(Plasmid #222117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
  • Backbone manufacturer
    Marco Jost & Jonathan Weissman, Addgene plasmid #188899
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-IRES-mCherry
  • Species
    Synthetic
  • Mutation
    AUC start codon

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP-AUC-IRES-mCherry was a gift from Jonathan Weissman (Addgene plasmid # 222117 ; http://n2t.net/addgene:222117 ; RRID:Addgene_222117)

  • For your References section:

    Translational fidelity screens in mammalian cells reveal eIF3 and eIF4G2 as regulators of start codon selectivity. She R, Luo J, Weissman JS. Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 10.1093/nar/gkad329. 10.1093/nar/gkad329 PubMed 37144468
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