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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry
(Plasmid #222107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAVS1
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPRi-kox1(KRAB)
  • Alt name
    dCas9-KRAB
  • Species
    H. sapiens (human); S. pyogenes
  • Mutation
    D10A, H840A
  • Entrez Gene
    cas9 (a.k.a. DQM35_RS05165, NCTC12064_01025, csn1)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • P2A-mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Catalytically deactivated Cas9

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1_dCas9-XTEN-KRAB-p2A-mCherry was a gift from Jonathan Weissman (Addgene plasmid # 222107 ; http://n2t.net/addgene:222107 ; RRID:Addgene_222107)

  • For your References section:

    Comparative landscape of genetic dependencies in human and chimpanzee stem cells. She R, Fair T, Schaefer NK, Saunders RA, Pavlovic BJ, Weissman JS, Pollen AA. Cell. 2023 Jul 6;186(14):2977-2994.e23. doi: 10.1016/j.cell.2023.05.043. Epub 2023 Jun 20. 10.1016/j.cell.2023.05.043 PubMed 37343560
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