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Addgene

pCF1135
(Plasmid #222106)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222106 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBSJL2
  • Backbone size (bp) 7769
  • Modifications to backbone
    The tetM and bla antibiotic resistance cassettes and f1 phage origin were replaced with the cat gene and promoter from pRPF185 (Addgene #106367).
  • Vector type
    Bacterial Expression ; E. coli-Veillonella shuttle plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    In Veillonella strains, the plasmid can be selected with thiamphenicol (15 µg/mL)
  • Copy number
    Unknown

Cloning Information

  • Cloning method Other

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was constructed using parts of two other plasmids:
pBSJL2 (derived from pBSJL1; Addgene #71261) and pRPF185 (Addgene #106367)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF1135 was a gift from J Christopher Fenno (Addgene plasmid # 222106 ; http://n2t.net/addgene:222106 ; RRID:Addgene_222106)

  • For your References section:

    Development of a small shuttle plasmid for use in oral Veillonella and initial appraisal of potential for fluorescence-based applications. Goetting-Minesky MP, Kim J, White DT, Hayashi M, Rickard AH, Fenno JC. Lett Appl Microbiol. 2024 Aug 5;77(8):ovae069. doi: 10.1093/lambio/ovae069. 10.1093/lambio/ovae069 PubMed 39020263
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