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Addgene

pKL2299A
(Plasmid #222105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKL2299
  • Backbone size w/o insert (bp) 29605
  • Vector type
    Bacterial Expression, Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    virA
  • Species
    Agrobacterium tumefaciens
  • Insert Size (bp)
    2490
  • GenBank ID
    AAZ50512.1
  • Promoter virA promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTTCGGTCAAGGTTCTGGA
  • 3′ sequencing primer TTTCAACCAAACGACCGGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL2299A was a gift from Kan Wang (Addgene plasmid # 222105 ; http://n2t.net/addgene:222105 ; RRID:Addgene_222105)

  • For your References section:

    Enhancing Agrobacterium-mediated plant transformation efficiency through improved ternary vector systems and auxotrophic strains. Aliu E, Ji Q, Wlazlo A, Grosic S, Azanu MK, Wang K, Lee K. Front Plant Sci. 2024 Jul 23;15:1429353. doi: 10.3389/fpls.2024.1429353. eCollection 2024. 10.3389/fpls.2024.1429353 PubMed 39109064
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