LHZ1493
(Plasmid
#222102)
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Purpose(Empty Backbone) Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneARS1/CEN5
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Modifications to backboneContains SlugCas9-HF with mutations R247A, N415A, T421A and R656A.
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Vector typeYeast Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LHZ1493 was a gift from Yongming Wang (Addgene plasmid # 222102 ; http://n2t.net/addgene:222102 ; RRID:Addgene_222102)