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Addgene

pVP143
(Plasmid #222080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVP075
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Originally cloned in NEB-10beta cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
  • Species
    Synthetic

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rodrigues et al., 2020 (doi.org/10.1073/pnas.2013338118)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sfGFP and scaffold was amplified from: pEN-L1-PJ23119-Bsa1-PglpT-sfGFP-TrrfB-Bsa1-Scaf-L2.
sacB was amplified from: pEN-R2-SacB-L3.
The base editor expression unit was amplified from: pEN-L4-PvirB-dCas9-CDA-UL-T3T-R1.
AeBlue amplified from Addgene #117846.

Please visit https://doi.org/10.1101/2024.08.04.606528 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVP143 was a gift from Wayne Parrott (Addgene plasmid # 222080 ; http://n2t.net/addgene:222080 ; RRID:Addgene_222080)

  • For your References section:

    Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop new strains. Pennetti VJ, LaFayette PR, Parrott WA. bioRxiv 2024.08.04.606528 10.1101/2024.08.04.606528
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