pVP073
(Plasmid
#222076)
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Purpose(Empty Backbone) eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepGGP-AG
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Vector typeChromoprotein-marked pVS1 backbone
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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A portion of this plasmid was derived from a plasmid made byeforRed was synthesized.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.04.606528 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVP073 was a gift from Wayne Parrott (Addgene plasmid # 222076 ; http://n2t.net/addgene:222076 ; RRID:Addgene_222076) -
For your References section:
Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop new strains. Pennetti VJ, LaFayette PR, Parrott WA. bioRxiv 2024.08.04.606528 10.1101/2024.08.04.606528
