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pcDNA3-HA-human OCRL
(Plasmid #22207)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22207 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression, Bacterial Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oculocerebrorenal syndrome of Lowe
  • Alt name
    OCRL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • GenBank ID
    NM_000276
  • Entrez Gene
    OCRL (a.k.a. DENT2, Dent-2, INPP5F, LOCR, NPHL2, OCRL-1, OCRL1)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-human OCRL was a gift from Pietro De Camilli (Addgene plasmid # 22207 ; http://n2t.net/addgene:22207 ; RRID:Addgene_22207)

  • For your References section:

    A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway. Erdmann KS, Mao Y, McCrea HJ, Zoncu R, Lee S, Paradise S, Modregger J, Biemesderfer D, Toomre D, De Camilli P. Dev Cell. 2007 Sep . 13(3):377-90. 10.1016/j.devcel.2007.08.004 PubMed 17765681
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