pVP298
(Plasmid
#222067)
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Purpose(Empty Backbone) GreenGate DE base entry vector with an Esp3I domesticated backbone.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepGGD000
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Vector typeSynthetic Biology ; GreenGate compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsccdB Survival cells are NOT suitable for the unmodified plasmid containing the ccdB gene. The plasmid is provided in the recommended DB3.1 strain.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationDomesticated of Esp3I sites
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVP298 was a gift from Wayne Parrott (Addgene plasmid # 222067 ; http://n2t.net/addgene:222067 ; RRID:Addgene_222067) -
For your References section:
MultiGreen: A multiplexing architecture for GreenGate cloning. Pennetti VJ, LaFayette PR, Parrott WA. PLoS One. 2024 Sep 18;19(9):e0306008. doi: 10.1371/journal.pone.0306008. eCollection 2024. 10.1371/journal.pone.0306008 PubMed 39292669