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Addgene

pVP295
(Plasmid #222064)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222064 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGGA000
  • Vector type
    Synthetic Biology ; GreenGate compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    ccdB Survival cells are NOT suitable for the unmodified plasmid containing the ccdB gene. The plasmid is provided in the recommended DB3.1 strain.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    Domesticated of Esp3I sites

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVP295 was a gift from Wayne Parrott (Addgene plasmid # 222064 ; http://n2t.net/addgene:222064 ; RRID:Addgene_222064)

  • For your References section:

    MultiGreen: A multiplexing architecture for GreenGate cloning. Pennetti VJ, LaFayette PR, Parrott WA. PLoS One. 2024 Sep 18;19(9):e0306008. doi: 10.1371/journal.pone.0306008. eCollection 2024. 10.1371/journal.pone.0306008 PubMed 39292669
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