pVP096
(Plasmid
#222043)
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PurposepGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGGP-AG
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Vector typeSynthetic Biology ; GreenGate compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP1e
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene #160442.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVP096 was a gift from Wayne Parrott (Addgene plasmid # 222043 ; http://n2t.net/addgene:222043 ; RRID:Addgene_222043) -
For your References section:
MultiGreen: A multiplexing architecture for GreenGate cloning. Pennetti VJ, LaFayette PR, Parrott WA. PLoS One. 2024 Sep 18;19(9):e0306008. doi: 10.1371/journal.pone.0306008. eCollection 2024. 10.1371/journal.pone.0306008 PubMed 39292669