pVP083
(Plasmid
#222035)
-
Purpose(Empty Backbone) MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 222035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
* Log in to view industry pricing.
Backbone
-
Vector backbonepVP076
-
Vector typeSynthetic Biology ; GreenGate compatible cloning vector
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
MutationAll unused Esp3i sites are domesticated
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pVP083 was a gift from Wayne Parrott (Addgene plasmid # 222035 ; http://n2t.net/addgene:222035 ; RRID:Addgene_222035) -
For your References section:
MultiGreen: A multiplexing architecture for GreenGate cloning. Pennetti VJ, LaFayette PR, Parrott WA. PLoS One. 2024 Sep 18;19(9):e0306008. doi: 10.1371/journal.pone.0306008. eCollection 2024. 10.1371/journal.pone.0306008 PubMed 39292669