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Addgene

mBEN 1957
(Plasmid #22203)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone size w/o insert (bp) 7650
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stable 3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    BEN
  • Insert Size (bp)
    55
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer U6
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The hairpin sequence is
5'TGC GAG CAA CAG CAT CCA GTT TCA AGA GAA CTG GAT GCT GTT GCT CGC TTT TTT C 3'

May contain a GFP cassette or a puro cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mBEN 1957 was a gift from Ananda Roy (Addgene plasmid # 22203 ; http://n2t.net/addgene:22203 ; RRID:Addgene_22203)

  • For your References section:

    Determination and functional analysis of the consensus binding site for TFII-I family member BEN, implicated in Williams-Beuren syndrome. Lazebnik MB, Tussie-Luna MI, Roy AL. J Biol Chem. 2008 Apr 25. 283(17):11078-82. 10.1074/jbc.C800049200 PubMed 18326499
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