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Addgene

mRFP-human APPL1
(Plasmid #22202)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22202 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif
  • Alt name
    APPL1
  • Alt name
    DIP13alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2131
  • GenBank ID
    NM_012096
  • Entrez Gene
    APPL1 (a.k.a. APPL, DIP13alpha, MODY14)
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-human APPL1 was a gift from Pietro De Camilli (Addgene plasmid # 22202 ; http://n2t.net/addgene:22202 ; RRID:Addgene_22202)

  • For your References section:

    A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway. Erdmann KS, Mao Y, McCrea HJ, Zoncu R, Lee S, Paradise S, Modregger J, Biemesderfer D, Toomre D, De Camilli P. Dev Cell. 2007 Sep . 13(3):377-90. 10.1016/j.devcel.2007.08.004 PubMed 17765681
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