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Addgene

pGEX-N-TEV-Twin-Strep
(Plasmid #222014)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222014 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Cytiva
  • Backbone size (bp) 3679
  • Modifications to backbone
    The TEV cleavable site (ENLYFQG) and Twin-strep tag were inserted instead of GST protein.
  • Vector type
    Bacterial Expression
  • Promoter lac promoter
  • Tags / Fusion Proteins
    • TEV cleavable site (ENLYFQG) (C terminal on backbone)
    • Twin-strep-tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATTAGGCACCCCAGGCTTTACACTTTATG
  • 3′ sequencing primer GCTTACAGACAAGCTGTGACCGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid can be linearized using the restriction enzyme (XhoI).
DNA (CDS) can be inserted through in-fusion cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-N-TEV-Twin-Strep was a gift from Chiaki Murakami (Addgene plasmid # 222014 ; http://n2t.net/addgene:222014 ; RRID:Addgene_222014)
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