pSFG-TFII-I-GFP beta
(Plasmid
#22200)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22200 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSFG
- Backbone size w/o insert (bp) 8900
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTFII-I
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Alt nameTFII-I beta isoform
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SpeciesH. sapiens (human)
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Insert Size (bp)2900
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Entrez GeneGTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector was derived from pSFG-tcLuc (Lindemann et al., 1998).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSFG-TFII-I-GFP beta was a gift from Ananda Roy (Addgene plasmid # 22200 ; http://n2t.net/addgene:22200 ; RRID:Addgene_22200) -
For your References section:
Opposing functions of TFII-I spliced isoforms in growth factor-induced gene expression. Hakre S, Tussie-Luna MI, Ashworth T, Novina CD, Settleman J, Sharp PA, Roy AL. Mol Cell. 2006 Oct 20. 24(2):301-8. 10.1016/j.molcel.2006.09.005 PubMed 17052463
Map uploaded by the depositor.
