pEGFPN1-human Dynamin 1aa K44A
(Plasmid #22197)

• Depositing Lab: Pietro De Camilli
• Publication: Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99(1):161-6.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Dynamin 1aa K44A
• Alt name: dyn1 K44A
• Alt name: DNM1 K44A
• Species: H. sapiens (human)
• Insert Size (bp): 2595
• Mutation: K44A
• GenBank ID: AAA02803.1
• Entrez Gene: DNM1 (a.k.a. DEE31, DNM, EIEE31)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII/ BamHI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primer
• 3′ sequencing primer: EGFP-C Sequencing Primer

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry