pSFG-TFII-I-GFP Y248&249F
(Plasmid #22196)

• Depositing Lab: Ananda Roy
• Publication: Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-52.

Backbone

• Vector backbone: pSFG
• Backbone size w/o insert (bp): 8900
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TFII-I
• Alt name: TF-II-I delta isoform
• Species: H. sapiens (human)
• Insert Size (bp): 2900
• Mutation: Changed Tyrosines 248 and 249 to Phenylalanines. Important: There may be a point mutation, Tyrosine 611 changed to Phenylalanine
• Entrez Gene: GTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector was derived from pSFG-tcLuc (Lindemann et al., 1998).

Important: There may be a point mutation, Tyrosine 611 changed to Phenylalanine