pRetroX-TRE3G_CBPdIDR7-GFP
(Plasmid
#221928)
-
PurposeDox-inducible expression of CBPdeltaIDR7-GFP in mammalian cells by retroviral transduction
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 221928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRetro-TRE3G
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6600
-
Vector typeRetroviral
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCBPdeltaIDR7
-
Alt nameCREB binding protein
-
SpeciesH. sapiens (human)
-
Insert Size (bp)7082
-
Mutationinsertion of CBP-GFP; deletion of IDR7 (aa 2109-2434)
-
Entrez GeneCREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
-
Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRetroX-TRE3G_CBPdIDR7-GFP was a gift from Daniel Bose (Addgene plasmid # 221928 ; http://n2t.net/addgene:221928 ; RRID:Addgene_221928)