pRetroX-TRE3G_CBP-GFP
(Plasmid #221926)

• Purpose: Dox-inducible expression of CBP-GFP in mammalian cells by retroviral transduction
• Depositing Lab: Daniel Bose
• Publication: Bose Lab CBP Expression Plasmids (unpublished)

Backbone

• Vector backbone: pRetro-TRE3G
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6600
• Vector type: Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CBP
• Alt name: CREB binding protein
• Species: H. sapiens (human)
• Insert Size (bp): 8060
• Mutation: insertion of CBP-GFP
• Entrez Gene: CREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pRetroX-TRE3G_CBP-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry