pHR-mCh-Cry2WT_NLS
(Plasmid
#221925)
-
PurposeExpression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 221925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHr-mCh-Cry2WT
-
Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 11303
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSV40 NLS
-
Alt nameCREB binding protein
-
SpeciesSynthetic
-
Insert Size (bp)112
-
Mutationinsertion of SV40 NLS
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-mCh-Cry2WT_NLS was a gift from Daniel Bose (Addgene plasmid # 221925 ; http://n2t.net/addgene:221925 ; RRID:Addgene_221925)