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Addgene

pHR-AL-mCh-Cry2WT-NLS
(Plasmid #221924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHr-mCh-Cry2WT
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 11303
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBP AL (aa 1529-1607)
  • Alt name
    CREB binding protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    236
  • Mutation
    insertion of the autoregulatory loop (AL) (aa 1529 - 1607); insertion of SV40 NLS
  • Entrez Gene
    CREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-AL-mCh-Cry2WT-NLS was a gift from Daniel Bose (Addgene plasmid # 221924 ; http://n2t.net/addgene:221924 ; RRID:Addgene_221924)
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