pHR-IDR1-mCh-Cry2WT-NLS
(Plasmid
#221918)
-
PurposeExpression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 221918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHr-mCh-Cry2WT
-
Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 11303
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCBP IDR1 (aa 72-175)
-
Alt nameCREB binding protein
-
SpeciesH. sapiens (human)
-
Insert Size (bp)312
-
Mutationinsertion of the IDR1 region (aa 72 - 175); insertion of SV40 NLS
-
Entrez GeneCREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-IDR1-mCh-Cry2WT-NLS was a gift from Daniel Bose (Addgene plasmid # 221918 ; http://n2t.net/addgene:221918 ; RRID:Addgene_221918)