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pAcGFP-N1_CBPdCFIDdAL
(Plasmid #221916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4726
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBP
  • Alt name
    CREB binding protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5559
  • Mutation
    deletion of the CBP-FUS interaction domain (CFID) (aa 1892-2441); deletion of the AL (aa 1558-1607); insertion at this site of a glycine-serine linker (GSAGSAAGSGQF)
  • Entrez Gene
    CREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGFP-N1_CBPdCFIDdAL was a gift from Daniel Bose (Addgene plasmid # 221916 ; http://n2t.net/addgene:221916 ; RRID:Addgene_221916)
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