pTER_MDC1 (422-2)
(Plasmid
#22191)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4358
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Vector typeMammalian Expression, RNAi
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesh Mediator of DNA damage checkpoint 1
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Alt nameshMDC1
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SpeciesH. sapiens (human)
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Insert Size (bp)58
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer H1
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTER_MDC1 (422-2) was a gift from Eric Campeau (Addgene plasmid # 22191 ; http://n2t.net/addgene:22191 ; RRID:Addgene_22191)