pAcGFP-N1_CBPdCFID
(Plasmid
#221909)
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PurposeTransient expression of GFP-tagged CBPdeltaCFID in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4726
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBPdeltaCFID
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Alt nameCREB binding protein
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SpeciesH. sapiens (human)
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Insert Size (bp)5674
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Mutationdeletion of the CBP-FUS interaction domain (CFID)(aa 1892-2441)
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Entrez GeneCREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcGFP-N1_CBPdCFID was a gift from Daniel Bose (Addgene plasmid # 221909 ; http://n2t.net/addgene:221909 ; RRID:Addgene_221909)