pEBG-TFII-I Y248&249F
(Plasmid #22189)

• Depositing Lab: Ananda Roy
• Publication: Novina et al J Biol Chem. 1998 Dec 11. 273(50):33443-8.

Backbone

• Vector backbone: pEBG
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TFII-I
• Species: H. sapiens (human)
• Insert Size (bp): 3400
• Mutation: Changed Tyrosines 248 and 249 to Phenylalanines.
• Entrez Gene: GTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)
• 5′ sequencing primer: EF-1a Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There may be a His tag N-terminal to the insert.