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TH1148-pOCC177-FUS(Nhe)-PLD_YtoF
(Plasmid #221887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOEM1
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1584
  • Mutation
    In PLD all Tyr (Y) changed to Phe (F)
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Promoter PH promoter
  • Tags / Fusion Proteins
    • 6xHis-MBP-PreScission (N terminal on insert)
    • TEV-SNAP-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TH1148-pOCC177-FUS(Nhe)-PLD_YtoF was a gift from Anthony Hyman (Addgene plasmid # 221887 ; http://n2t.net/addgene:221887 ; RRID:Addgene_221887)

  • For your References section:

    A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins. Wang J, Choi JM, Holehouse AS, Lee HO, Zhang X, Jahnel M, Maharana S, Lemaitre R, Pozniakovsky A, Drechsel D, Poser I, Pappu RV, Alberti S, Hyman AA. Cell. 2018 Jul 26;174(3):688-699.e16. doi: 10.1016/j.cell.2018.06.006. Epub 2018 Jun 28. 10.1016/j.cell.2018.06.006 PubMed 29961577
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