TH0994-pOCC177-Fus-wt_(1-211)
(Plasmid
#221886)
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PurposeShuttle vector for baculovirus production, using FlashBac bacmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOEM1
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFUS
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SpeciesH. sapiens (human)
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Insert Size (bp)633
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MutationPLD domain (aa 1-211)
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Entrez GeneFUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
- Promoter PH promoter
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Tags
/ Fusion Proteins
- 6xHis-MBP-PreScission (N terminal on insert)
- TEV-SNAP-tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TH0994-pOCC177-Fus-wt_(1-211) was a gift from Anthony Hyman (Addgene plasmid # 221886 ; http://n2t.net/addgene:221886 ; RRID:Addgene_221886) -
For your References section:
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins. Wang J, Choi JM, Holehouse AS, Lee HO, Zhang X, Jahnel M, Maharana S, Lemaitre R, Pozniakovsky A, Drechsel D, Poser I, Pappu RV, Alberti S, Hyman AA. Cell. 2018 Jul 26;174(3):688-699.e16. doi: 10.1016/j.cell.2018.06.006. Epub 2018 Jun 28. 10.1016/j.cell.2018.06.006 PubMed 29961577