TH0901-pOCC177-FUS-wt_opt(Nhe)
(Plasmid #221885)

• Purpose: Shuttle vector for baculovirus production, using FlashBac bacmid
• Depositing Lab: Anthony Hyman
• Publication: Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. doi: 10.1016/j.cell.2018.06.006. Epub 2018 Jun 28.

Backbone

• Vector backbone: pOEM1
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FUS
• Species: H. sapiens (human)
• Insert Size (bp): 1584
• Mutation: wt
• Entrez Gene: FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
• Promoter: PH promoter
• Tags / Fusion Proteins:
  • 6xHis-MBP-PreScission (N terminal on insert)
  • TEV-SNAP-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthetic

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry