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Addgene

pEBG-TFII-I-p70
(Plasmid #22187)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22187 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TFII-I
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    Contains only amino acids 1-735 C-terminal residues have been deleted.
  • Entrez Gene
    GTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer EF-1a Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG-TFII-I-p70 was a gift from Ananda Roy (Addgene plasmid # 22187 ; http://n2t.net/addgene:22187 ; RRID:Addgene_22187)

  • For your References section:

    TFII-I regulates Vbeta promoter activity through an initiator element. Cheriyath V, Novina CD, Roy AL. Mol Cell Biol. 1998 Aug . 18(8):4444-54. PubMed 9671454
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