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Addgene

pCMV-CRT-Fc N
(Plasmid #221854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Calreticulin
  • Species
    H. sapiens (human)
  • Mutation
    Expresses N domain
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Tag / Fusion Protein
    • Human IgG1Fc tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-CRT-Fc N was a gift from Judy Lieberman (Addgene plasmid # 221854 ; http://n2t.net/addgene:221854 ; RRID:Addgene_221854)

  • For your References section:

    The NK cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells. Sen Santara S, Lee DJ, Crespo A, Hu JJ, Walker C, Ma X, Zhang Y, Chowdhury S, Meza-Sosa KF, Lewandrowski M, Zhang H, Rowe M, McClelland A, Wu H, Junqueira C, Lieberman J. Nature. 2023 Apr;616(7956):348-356. doi: 10.1038/s41586-023-05912-0. Epub 2023 Apr 5. 10.1038/s41586-023-05912-0 PubMed 37020026
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