pCMV-CRT-Fc FL
(Plasmid
#221853)
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PurposeExpress human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecalreticulin
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SpeciesH. sapiens (human)
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Entrez GeneCALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
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Tag
/ Fusion Protein
- Human IgG1Fc tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-CRT-Fc FL was a gift from Judy Lieberman (Addgene plasmid # 221853 ; http://n2t.net/addgene:221853 ; RRID:Addgene_221853) -
For your References section:
The NK cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells. Sen Santara S, Lee DJ, Crespo A, Hu JJ, Walker C, Ma X, Zhang Y, Chowdhury S, Meza-Sosa KF, Lewandrowski M, Zhang H, Rowe M, McClelland A, Wu H, Junqueira C, Lieberman J. Nature. 2023 Apr;616(7956):348-356. doi: 10.1038/s41586-023-05912-0. Epub 2023 Apr 5. 10.1038/s41586-023-05912-0 PubMed 37020026
