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Addgene

Ub-LPNTG-pET
(Plasmid #221850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Species
    H. sapiens (human)
  • Mutation
    Arg74ProLeuThr

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ub-LPNTG-pET was a gift from Artur Krężel (Addgene plasmid # 221850 ; http://n2t.net/addgene:221850 ; RRID:Addgene_221850)

  • For your References section:

    Site-Specific and Fluorescently Enhanced Installation of Post-Translational Protein Modifications via Bifunctional Biarsenical Linker. Antonenko A, Pomorski A, Singh AK, Kapczynska K, Krezel A. ACS Omega. 2024 Oct 30;9(45):45127-45137. doi: 10.1021/acsomega.4c05828. eCollection 2024 Nov 12. 10.1021/acsomega.4c05828 PubMed 39554417
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