pEBG-TFII-I deltaBR
(Plasmid
#22185)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEBG
- Backbone size w/o insert (bp) 6000
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameTFII-I
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2900
-
MutationDeleted amino acids 301-306
-
Entrez GeneGTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
-
Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Acc65I (unknown if destroyed)
- 3′ cloning site SnaB1 (unknown if destroyed)
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There may be a His tag N-terminal to the insert.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEBG-TFII-I deltaBR was a gift from Ananda Roy (Addgene plasmid # 22185 ; http://n2t.net/addgene:22185 ; RRID:Addgene_22185) -
For your References section:
Structure-function analysis of TFII-I. Roles of the N-terminal end, basic region, and I-repeats. Cheriyath V, Roy AL. J Biol Chem. 2001 Mar 16. 276(11):8377-83. 10.1074/jbc.M008411200 PubMed 11113127
Map uploaded by the depositor.
