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Addgene

pET28-Butelase
(Plasmid #221848)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221848 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Butelase 1 ligase
  • Species
    Clitoria ternatea
  • Mutation
    No

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-Butelase was a gift from Artur Krężel (Addgene plasmid # 221848 ; http://n2t.net/addgene:221848 ; RRID:Addgene_221848)

  • For your References section:

    An efficient and easily obtainable butelase variant for chemoenzymatic ligation and modification of peptides and proteins. Singh AK, Antonenko A, Kocyla A, Krężel A. Microb Cell Fact. 2024 Nov 30;23(1):325. doi: 10.1186/s12934-024-02598-5. 10.1186/s12934-024-02598-5 PubMed 39614317
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